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Updated: Jul 11, 2026

A Mouse 5/6th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

A Mouse 5/6^th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

Published on: November 7, 2017

Collagen type VIII expression in human diabetic nephropathy.

J Gerth¹, C D Cohen, U Hopfer

¹University of Jena, Jena, Germany.

European Journal of Clinical Investigation

|September 25, 2007

Summary

Collagen type VIII is significantly upregulated in diabetic nephropathy, unlike other kidney diseases. This specific increase in collagen type VIII may drive diabetic nephropathy's progression by influencing cell proliferation and migration.

Related Experiment Videos

Last Updated: Jul 11, 2026

A Mouse 5/6th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

A Mouse 5/6^th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy

Published on: November 7, 2017

Area of Science:

Nephrology
Molecular Biology
Biochemistry

Background:

Collagen type VIII, a short-chain collagen, potentially influences cell migration, proliferation, and adherence.
Limited data exists on collagen type VIII expression in human diabetic nephropathy.

Purpose of the Study:

To investigate collagen type VIII (COL8A1 and COL8A2) mRNA and protein expression in human kidney biopsies.
To compare expression patterns in diabetic nephropathy versus normal kidneys, benign nephrosclerosis, and focal-segmental glomerulosclerosis.

Main Methods:

Retrospective analysis of 20 diabetic nephropathy biopsies, 16 benign nephrosclerosis, 9 FSGS, and 10 normal kidney biopsies.
Real-time PCR used to quantify COL8A1 and COL8A2 mRNA expression in glomerular and tubular compartments.
Immunohistochemistry and Western blotting employed to assess collagen type VIII protein levels and antibody specificity.

Main Results:

Significant induction of COL8A1 and COL8A2 mRNA was observed in both glomerular and tubular compartments of diabetic nephropathy kidneys.
COL8A1 mRNA expression was notably higher than COL8A2.
Increased collagen type VIII protein expression was detected in mesangial cells, tubules, and interstitium in diabetic nephropathy, with minimal staining in normal kidneys.

Conclusions:

Collagen type VIII upregulation is specific to diabetic nephropathy among the studied renal diseases.
The diabetic environment, not proteinuria or serum creatinine levels, likely drives collagen type VIII expression.
Elevated collagen type VIII may contribute to diabetic nephropathy pathogenesis by promoting cell proliferation and migration.