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Updated: Jul 11, 2026
Vitrification of Ovarian Cortex Tissue to Achieve a Glassy State of Aggregation
Published on: August 9, 2024
Morphologic, ultrastructural, and biochemical identification of apoptosis in vitrified-warmed mouse ovarian tissue
Tahere Mazoochi1, Mojdeh Salehnia, Mojtaba Rezazadeh Valojerdi
1Department of Anatomy, Tarbiat Modares University, Tehran, Iran.
Objective:
To evaluate the incidence of apoptosis after vitrification warming of mouse ovaries.
Design:
Experimental study.
Setting:
University-based research laboratory.
Animal(S):
Twelve- to 14-day-old National Medical Research Institute female mice.
Intervention(S):
Vitrification of mouse ovaries using ethylene glycol.
Main Outcome Measure(S):
Follicle viability assessment by trypan blue testing, morphologic examination by hematoxylin-eosin staining and transmission electron microscopy, apoptosis assessment using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling method and DNA laddering technique.
Result(S):
No statistically significant difference in follicle viability was observed between vitrified and nonvitrified ovaries. On transmission electron microscopy, vitrified ovaries showed a well-preserved ultrastructure. No sign of apoptosis was observed morphologically or by transferase-mediated deoxyuridine triphosphate nick end-labeling technique in either fresh or vitrified-warmed mouse ovaries. Despite the presence of a laddering pattern of DNA in control induced thymic tissue, no similar pattern was observed in fresh or vitrified-warmed ovaries.
Conclusion(S):
The data suggest that the vitrification technique does not induce apoptosis in mouse ovarian tissue investigated just after warming.
