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Updated: Aug 9, 2026
15:06
Synthesis of an Intein-mediated Artificial Protein Hydrogel
Published on: January 27, 2014
[Enzymatic hydrolysis of gelatin]
Ukrainskii Biokhimicheskii Zhurnal (1978)
|September 1, 1991
Summary
Enzyme hydrolysis effectively degrades gelatin, enhancing ultrafiltration membrane performance. Pronase-1 and industrial proteases offer optimal gelatin destruction for membrane recovery.
Area of Science:
- Biochemistry
- Enzymology
- Membrane Science
Context:
- Ultrafiltration membranes are crucial in various industrial processes.
- Gelatin fouling can impair membrane permeability and selectivity.
- Enzymatic hydrolysis offers a potential solution for membrane cleaning.
Purpose:
- To investigate enzymatic hydrolysis of gelatin using proteolytic enzymes.
- To identify the most effective enzymes for gelatin macromolecule degradation.
- To restore the permeability and selective properties of ultrafiltration membranes.
Summary:
- Gelatin hydrolysis was studied using proteolytic enzymes to assess their effectiveness in degrading gelatin macromolecules.
- The Lee and Takahashi method, with enhanced precision (6-8%), quantified free alpha-NH2-groups to determine the degree of gelatin destruction.
- The enzyme preparation "Pronaz-1" and industrial enzymes like alkali protease and protease C demonstrated maximum gelatin destruction (48.2% within 1-2 hours).
Impact:
- Identified optimal enzyme preparations for efficient gelatin hydrolysis.
- Provides a method for restoring ultrafiltration membrane functionality.
- Contributes to improved membrane maintenance and longevity in industrial applications.
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