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Updated: Aug 9, 2026

Synthesis of an Intein-mediated Artificial Protein Hydrogel
Published on: January 27, 2014
[Enzymatic hydrolysis of gelatin]
Abstract:
The process of gelatin hydrolysis by means of enzymes of the proteolytic action with the aim to determine most effective destructor of gelatin macromolecules for recovering permeability and selective properties of ultrafiltration membranes was investigated. The presence of free alpha-NH2-groups was determined by means of the Lee and Takahashi method. Calculation of the destruction degree of substances in the Lee and Takahashi method during determination of the quantity of free alpha-NH2-groups rose precision of the method by 6-8%. The maximum degree of destruction (48.2% for 1-2 hours) was provided by the enzyme preparation "Pronaz-1" (Str. griseus + Acr. chrysogenum) and by industrial enzymes: alkali proteaze and proteaze C.
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