Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

OMP decarboxylase--An enigma persists.

Brian P Callahan1, Brian G Miller

  • 1Division of Infectious Disease, Wadsworth Center--NYS-DOH, Albany, NY 12202, USA. callahan@wadsworth.org

Bioorganic Chemistry
|September 25, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phylogenetic investigation of the 100 kDa hexokinase enzyme family with the topiary ancestral sequence reconstruction pipeline.

Protein science : a publication of the Protein Society·2026
Same author

Conformational Diversity and Substrate Specificity are Decoupled in Ancestral and Extant Glucokinases.

bioRxiv : the preprint server for biology·2026
Same author

Conformational Expansion Underlies the Evolutionary Emergence of Redox Sensitivity in Vertebrate Glucokinases.

bioRxiv : the preprint server for biology·2026
Same author

Structural Co-optation and Loss-of-function Underlie the Evolution of Regulatory Novelty in the Glucokinase Regulatory Protein.

bioRxiv : the preprint server for biology·2026
Same author

Evolution of Protein Regulation in the Vertebrate Glucose Sensor.

bioRxiv : the preprint server for biology·2026
Same author

In vitro reconstitution of vertebrate Sonic Hedgehog protein cholesterolysis.

bioRxiv : the preprint server for biology·2026
Same journal

Tumor-targeted activatable molecular agent for synergistic chemo-photodynamic therapy.

Bioorganic chemistry·2026
Same journal

Computational design and experimental validation of peptide inhibitors targeting the p53-COP1 interaction to suppress p53 ubiquitination.

Bioorganic chemistry·2026
Same journal

Benzalkonium chloride disrupts human placental progesterone synthesis via locking NAD<sup>+</sup> pocket in 3β-Hydroxysteroid dehydrogenase 1 and rat homolog: docking evidence and 3D-QSAR, and experiment risk assessment.

Bioorganic chemistry·2026
Same journal

Terephthaloyl-based amide-linked derivatives and polymeric networks with selective cytotoxicity: In vitro evaluation, Molecular docking, Molecular dynamics, and DFT insights.

Bioorganic chemistry·2026
Same journal

Synthesis and biological evaluation of C6-modified Celastrol derivatives as anti-inflammatory agents targeting the NF-κB signaling pathway.

Bioorganic chemistry·2026
Same journal

Synthesis and biological evaluation of 6,9-disubstituted purine analogues inducing autophagy-mediated apoptosis and potentiating carboplatin response in high-grade serous ovarian cancer.

Bioorganic chemistry·2026
See all related articles

Orotidine 5'-phosphate decarboxylase (ODCase) is the most effective protein catalyst known, with a rate enhancement of 10(17). This perspective reviews proposed reaction pathways and experimental data to understand its catalytic power.

Area of Science:

  • Biochemistry
  • Enzymology
  • Chemical Catalysis

Background:

  • Orotidine 5 -phosphate decarboxylase (ODCase) exhibits an extraordinary rate enhancement of approximately 10(17).
  • This makes ODCase the most potent pure protein catalyst discovered to date.
  • Numerous hypotheses have been proposed over the past 12 years to elucidate this remarkable catalytic efficiency.

Purpose of the Study:

  • To summarize and critically evaluate proposed reaction pathways for ODCase.
  • To highlight experimental evidence supporting or refuting these pathways.
  • To suggest future experiments for further investigation of ODCase's mechanism.

Main Methods:

  • Literature review of existing hypotheses and experimental data.
  • Analysis of supporting and refuting evidence for proposed ODCase reaction mechanisms.

Related Experiment Videos

  • Proposal of new experimental designs to test candidate pathways.

    • Main Results:

    • A comprehensive overview of current hypotheses regarding ODCase's catalytic mechanism.
    • Identification of key experimental findings that support or contradict proposed pathways.
    • Recommendations for targeted experiments to differentiate between proposed mechanisms.

    Conclusions:

    • Understanding the mechanism of ODCase is crucial for enzyme catalysis research.
    • Further experimental validation is required to definitively determine the reaction pathway.
    • This work provides a framework for future research into highly efficient enzymatic catalysis.