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Related Experiment Video

Updated: Jul 11, 2026

Embryo Transfer Surgery via Laparotomy in Gilts

Embryo Transfer Surgery via Laparotomy in Gilts

Published on: October 18, 2024

Piglets born from handmade cloning, an innovative cloning method without micromanipulation.

Y Du¹, P M Kragh, Y Zhang

¹Population Genetics and Embryology, Insitute of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, DK-8830 Tjele, Denmark. Yutao.Du@agrsci.dk

|September 25, 2007

Summary

Porcine handmade cloning (HMC) achieved its first healthy piglet births, demonstrating biological value. This simplified cloning method shows promise for efficient swine reproduction and genetic applications.

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Area of Science:

Animal Science
Reproductive Biology
Biotechnology

Background:

Traditional cloning (TC) in pigs relies on complex micromanipulation.
Porcine handmade cloning (HMC) offers a simplified alternative, but live offspring had not been achieved.
Optimizing HMC is crucial for its practical application in swine reproduction.

Purpose of the Study:

To report the first successful birth of healthy piglets using porcine handmade cloning (HMC).
To evaluate the efficiency and developmental rates of embryos produced via HMC.
To demonstrate the biological viability of HMC for producing swine offspring.

Main Methods:

Optimized manual procedures for embryo reconstruction in HMC, including fusion and activation steps.
In vitro culture (IVC) of HMC-produced embryos to the blastocyst stage.
Surgical transfer of HMC-derived blastocysts into synchronized recipient sows.

Main Results:

Achieved a high blastocyst development rate of 50.1% from HMC-reconstructed embryos, with an average cell number of 77.
Reported the birth of healthy piglets from HMC, with a live birth efficiency of 17.2% (10/58) for HMC embryos.
Observed the highest in vitro developmental rate to date for porcine somatic cell nuclear transfer (SCNT).

Conclusions:

Porcine handmade cloning (HMC) can successfully produce healthy piglets, validating its biological value.
HMC represents a promising, simplified approach for swine cloning with potential for large-scale application.
Further in vivo studies are necessary to fully stabilize and optimize the HMC technique for broader use.