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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
Isolation of microRNA targets using biotinylated synthetic microRNAs
Ulf Andersson Orom1, Anders H Lund
1Biotech Research & Innovation Centre, Ole Maaløes Vej 5, 2200 Copenhagen, Denmark.
Methods (San Diego, Calif.)
|September 25, 2007
Abstract:
MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets.
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