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Updated: Jul 11, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Extracting kinetic rate constants from surface plasmon resonance array systems.

Rebecca L Rich¹, Michelle J Cannon, Jerry Jenkins

¹Center for Biomolecular Interaction Analysis, School of Medicine, University of Utah, Salt Lake City, UT 84132, USA.

Analytical Biochemistry

|September 25, 2007

Summary

This summary is machine-generated.

Surface plasmon resonance imaging (SPR) systems can analyze many reactions at once. This study shows a model to accurately measure binding kinetics, even with mass transport effects, reducing variability to under 9%.

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Area of Science:

Biophysics
Analytical Chemistry
Biotechnology

Background:

Surface plasmon resonance imaging (SPR) systems enable high-throughput analysis of biomolecular interactions.
Large-format SPR flow cells present challenges like mass transport effects and sample depletion, complicating kinetic analysis.
Accurate interpretation of binding kinetics is crucial for understanding molecular interactions in SPR assays.

Purpose of the Study:

To characterize mass transport and sample depletion effects in large-format SPR flow cells.
To develop and validate a computational model for accurate kinetic analysis in SPR arrays.
To assess and minimize spot-to-spot variability in SPR binding kinetics measurements.

Main Methods:

Utilized a combination of computational simulations and experimental SPR imaging.
Employed a two-compartment model to analyze binding data across spotted arrays.
Validated the model using antibody-antigen interaction systems.

Main Results:

Established that a simple two-compartment model accurately extracts intrinsic rate constants under mass transport-limited conditions.
Demonstrated <9% spot-to-spot variability in binding kinetics for antibody systems.
Showcased the benefits of global fitting for mass transport-limited SPR data.

Conclusions:

The two-compartment model effectively addresses challenges in large-format SPR kinetic analysis.
SPR imaging systems can provide reliable and reproducible binding kinetic data with proper modeling.
Global fitting of SPR data enhances accuracy for mass transport-limited systems.