Related Experiment Video
Updated: Jul 11, 2026

Demonstrating a Linear Relationship Between Vascular Endothelial Growth Factor and Luteinizing Hormone in Kidney Cortex Extracts
Published on: January 22, 2020
Ovarian vascular endothelial growth factor and vascular endothelial growth factor receptor patterns in reproductive
John Yeh1, Beom Su Kim, Jennifer Peresie
1Department of Gynecology-Obstetrics, University at Buffalo, State University of New York, Buffalo, New York 14222, USA. jyeh@buffalo.edu
Objective:
To investigate how levels of vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) change during corpus luteum formation in reproductive aging.
Design:
Adult and reproductive aging female rats were given pregnant mare serum gonadotropin (PMSG) and the ovaries and sera were collected at 0, 3, 5, and 7 days after human chorionic gonadotropin (hCG) injection.
Setting:
Laboratory study.
Patient(S):
Animal model.
Intervention(S):
Induction of corpus luteum formation with PMSG-hCG.
Main Outcome Measure(S):
Western blot analysis for ovarian VEGF, VEGFR-1, VEGFR-2, and hypoxia-induced factor 1 (HIF1-alpha). Enzyme-linked immunosorbent assay (ELISA) measurement of serum VEGF levels.
Result(S):
For VEGF and VEGFR-1, there was no difference between the adult and the reproductive aging ovaries. For total VEGFR-2 levels, the adult ovaries had a decline during corpus luteum formation. In contrast, the levels of the two forms of phosphorylated VEGFR-2 declined in aging ovaries. There was a decline in HIF1-alpha during corpora lutea formation in the reproductive aging rats.
Conclusion(S):
The reproductive aging corpora lutea have a temporal decline in phosphorylated VEGFR-2 levels but not in the total VEGFR-2 levels. The data suggest that the VEGFR response mechanism is different in the aging ovary.
More Related Videos
08:05Doppler Ultrasonography for Live Imaging and Quantification of Ovarian Vascular Function in Mice
Published on: November 14, 2025
07:40Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery
Published on: May 16, 2018
Related Concept Videos
Regulation of Angiogenesis and Blood Supply
Oogenesis
Menopause