Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 11, 2026

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
10:19

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

Prion removal by nanofiltration under different experimental conditions.

Mikihiro Yunoki1, Hiroyuki Tanaka, Takeru Urayama

  • 1Department of Virology, Research Institute for Microbial Diseases, Osaka University, Japan. yunoki.mikihiro@mk.m-pharma.co.jp

Biologicals : Journal of the International Association of Biological Standardization
|September 25, 2007
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perinatal social work during the Covid-19 pandemic: Reflecting on concepts of time and liminality.

Qualitative social work : QSW : research and practice·2021
Same author

A comprehensive microbiological safety approach for agarose encapsulated porcine islets intended for clinical trials.

Xenotransplantation·2016
Same author

Extracorporeal membrane oxygenation with double venous drainage.

Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia·2013
Same author

Japanese growth prediction model for prepubertal children with growth hormone deficiency.

Journal of pediatric endocrinology & metabolism : JPEM·2013
Same author

Diagnosis of structural deterioration in an aortic bioprosthetic valve by multidetector row computed tomography: report of a case.

Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia·2013
Same author

Novel and recurrent PORCN gene mutations in almost unilateral and typical focal dermal hypoplasia patients.

European journal of dermatology : EJD·2013

Filter efficacy against prion removal depends on the preparation of the spiked material. Optimized prion preparations require smaller filter pore sizes (15 nm) for effective removal, highlighting the importance of sample preparation in TSE agent clearance studies.

Area of Science:

  • Biopharmaceutical Manufacturing
  • Prion Disease Research
  • Filtration Technology

Background:

  • Transmissible spongiform encephalopathy (TSE) agents pose a risk in biopharmaceutical production.
  • Effective removal of prion protein (PrP Sc) is crucial for ensuring product safety.
  • Virus removal filters are commonly used, but their efficacy against prions needs thorough evaluation.

Purpose of the Study:

  • To assess the removal of scrapie prion protein (PrP Sc) using virus removal filters.
  • To investigate the impact of different starting material preparations on prion removal efficiency.
  • To determine the optimal filter pore size for PrP Sc clearance in bioprocessing.

Main Methods:

  • Evaluation of PrP Sc removal using virus filters with varying pore sizes (75 nm and 15 nm).

More Related Videos

High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
07:50

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Published on: December 8, 2017

Related Experiment Videos

Last Updated: Jul 11, 2026

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing
10:19

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
07:50

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Published on: December 8, 2017

  • Utilized hamster-adapted scrapie strain 263K (263K MF) as spike material, including untreated and 'super-sonicated' preparations.
  • Western blot (WB) analysis was employed to detect PrP Sc in filtrates.

    • Main Results:

    • PrP Sc was detected after 75 nm filtration with 'super-sonicated' 263K MF, but not with untreated 263K MF.
    • 15 nm filtration effectively removed PrP Sc below detection limits when spike materials had optimized particle size distribution.
    • Infectious PrP Sc was recovered in one instance following 15 nm filtration, indicating variability.

    Conclusions:

    • The preparation method of prion spike material significantly influences filter removal efficiency.
    • Minimizing prion particle size through sonication or detergent treatment is essential for accurate filter evaluation.
    • Careful consideration of spike preparation is necessary for validating prion clearance in biopharmaceutical manufacturing.