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Updated: Jul 11, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Genomic diversity in Staphylococcus xylosus.

Emilie Dordet-Frisoni¹, Géraud Dorchies, Cécilia De Araujo

¹INRA, Centre de Clermont-Ferrand Theix, Unité Microbiologie, Qualité et Sécurité des Aliments, 63122 Saint-Genès Champanelle, France.

Applied and Environmental Microbiology

|September 25, 2007

Summary

Staphylococcus xylosus strains show genetic diversity, with some potentially hazardous strains forming a distinct cluster. This diversity is concentrated near the chromosome

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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

Area of Science:

Microbiology
Genomics
Bacterial Pathogenesis

Background:

Staphylococcus xylosus is a common skin commensal and food bacterium.
While generally used in starter cultures for meat fermentation, some strains can cause opportunistic infections in animals.
Understanding intraspecies genetic diversity is crucial for distinguishing commensal from potentially hazardous strains.

Purpose of the Study:

To investigate the genetic diversity within Staphylococcus xylosus.
To identify genetic differences between commensal and potentially hazardous strains.
To pinpoint regions of the genome responsible for strain-specific characteristics and ecological fitness.

Main Methods:

Suppressive and subtractive hybridization (SSH) was employed to compare genetic material.
A commensal human skin strain (S. xylosus C2a) was used as a driver against three tester strains with different origins (starter culture, cow mastitis, mouse dermatitis).
Tester-specific DNA fragments were identified and analyzed, followed by distribution analysis across 20 S. xylosus strains.

Main Results:

SSH identified 122 tester-specific fragments, corresponding to 149 open reading frames (ORFs), many related to specific metabolic functions.
Analysis revealed that S. xylosus strains could be divided into two clusters, one exclusively comprising potentially hazardous strains.
Genetic diversity was found to be localized in a specific region near the chromosome's origin of replication.

Conclusions:

Staphylococcus xylosus exhibits significant intraspecies genetic diversity, with a distinct genetic makeup in potentially hazardous strains.
The identified region of genetic diversity, near the origin of replication, may represent a 'region of speciation' within the species.
This strain-specific region likely plays a role in the ecological fitness and pathogenic potential of different S. xylosus strains.