Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sortagging: a versatile method for protein labeling.

Maximilian W Popp1, John M Antos, Gijsbert M Grotenbreg

  • 1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.

Nature Chemical Biology
|September 25, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amino Acid Variants at the P94 Position in <i>Staphylococcus aureus</i> Class a Sortase Modulate Substrate Binding and Enzyme Activity.

Biochemistry·2026
Same author

Amino acid variants at the P94 position in <i>Staphylococcus aureus</i> class A sortase modulate substrate binding and enzyme activity.

bioRxiv : the preprint server for biology·2026
Same author

A Putative Interaction between the Transmembrane Domains of <i>Streptococcus pyogenes</i> Sortase A and Its Endogenous Substrate M Protein Revealed by Molecular Dynamics Simulations.

The journal of physical chemistry. B·2025
Same author

A modular Uba1-nanobody fusion enables selective ubiquitin transfer to tagged E2 enzymes.

The Journal of biological chemistry·2025
Same author

A multivalent nanobody-drug conjugate to prevent and treat influenza virus infections.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

An interaction between the transmembrane domains of <i>Streptococcus pyogenes</i> sortase A and its endogenous substrate M protein revealed by molecular dynamics simulations.

bioRxiv : the preprint server for biology·2025
Same journal

Cryo-EM sheds light on the mechanism of human telomerase inhibition by BIBR1532.

Nature chemical biology·2026
Same journal

Artificial metalloenzymes in complex biological environments.

Nature chemical biology·2026
Same journal

Allosteric disordering of eIF2B regulates the integrated stress response.

Nature chemical biology·2026
Same journal

A tail of two ligases.

Nature chemical biology·2026
Same journal

Non-canonical cytochrome P450 enzymes expand the diversity of bacterial hemoproteins.

Nature chemical biology·2026
Same journal

Image-guided activation of drugs with electromagnetic radiation.

Nature chemical biology·2026
See all related articles

Sortagging offers precise, versatile protein labeling for cell biology research. This chemoenzymatic method overcomes limitations of fluorescent reporters for in vitro and in vivo applications.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Genetically encoded fluorescent reporters are vital for observing cellular processes but have inherent limitations.
  • Existing protein labeling techniques often lack precision, versatility, or ease of use.

Purpose of the Study:

  • To apply sortagging (sortase-mediated transpeptidation) for site-specific protein labeling.
  • To demonstrate the utility of sortagging for labeling proteins both in vitro and on the surface of living cells.

Main Methods:

  • Utilized sortase enzymes for site-specific peptide bond formation.
  • Employed genetically encoded tags for precise enzymatic recognition.
  • Integrated chemical synthesis of small (<2 kDa) probes for versatile labeling.

Related Experiment Videos

Main Results:

  • Successfully achieved site-specific labeling of proteins using the sortagging system.
  • Demonstrated the applicability of sortagging for labeling proteins in cell-free systems (in vitro).
  • Showcased sortagging's effectiveness for labeling proteins on the surface of live cells.

Conclusions:

  • Sortagging provides a powerful chemoenzymatic approach for protein labeling.
  • This method combines genetic encoding precision with enzymatic specificity and chemical versatility.
  • Sortagging overcomes limitations of traditional fluorescent reporters for diverse cell biology applications.