Related Experiment Videos
Enterovirus type 70 virion and intracellular proteins
Journal of Virology
|June 1, 1976
Abstract:
The proteins of purified enterovirus type 70 grown in rhabdomyosarcoma cells and the intracellular proteins at 4 to 5 h after infection have been examined by polyacrylamide gel electrophoresis. Virions contained four proteins: P-1 (35,000, daltons) P-2 (28,000, daltons) P-3 (27,000, daltons) and P-4 (9,000 daltons). Further, addition of ZnCl2 to infected cultures inhibited virus plaque development and interfered with post-translational cleavage.
Insights
Enterovirus type 70 virions contain four distinct proteins. Zinc chloride addition to infected cells inhibits virus plaque development and post-translational cleavage, impacting viral replication.
Area of Science:
- Virology
- Molecular Biology
- Biochemistry
Background:
- Enterovirus type 70 (EV-70) is a significant human pathogen.
- Understanding the viral protein composition and replication mechanisms is crucial for developing antiviral strategies.
Purpose of the Study:
- To characterize the protein components of purified EV-70 virions.
- To investigate the effect of zinc chloride on EV-70 replication and protein processing.
Main Methods:
- Polyacrylamide gel electrophoresis (PAGE) was used to analyze viral and intracellular proteins.
- EV-70 was propagated in rhabdomyosarcoma cells.
- The impact of zinc chloride (ZnCl2) on virus plaque development was assessed.
Main Results:
- Purified EV-70 virions were found to comprise four proteins: P-1 (35 kDa), P-2 (28 kDa), P-3 (27 kDa), and P-4 (9 kDa).
- Addition of ZnCl2 to infected cell cultures significantly inhibited virus plaque formation.
- ZnCl2 treatment also interfered with the post-translational cleavage of viral proteins.
Conclusions:
- The study identifies the specific protein subunits of the enterovirus type 70 virion.
- Zinc chloride emerges as a potential inhibitor of EV-70 replication by disrupting viral protein processing and plaque development.