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Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...

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Related Experiment Video

Updated: Jul 11, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

DNA methylation and systemic lupus erythematosus.

Eva Balada1, Josep Ordi-Ros, Miquel Vilardell-Tarrés

  • 1Research Unit in Systemic Autoimmune Diseases, Vall d'Hebron Research Institute, Hospital Vall d'Hebron, Barcelona, Spain. ebalada@ir.vhebron.net

Annals of the New York Academy of Sciences
|September 26, 2007
PubMed
Summary

DNA hypomethylation is crucial in systemic lupus erythematosus (SLE). This study analyzes DNA methylation enzymes in SLE patients, exploring potential causes for global DNA hypomethylation in CD4+ T cells.

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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Related Experiment Videos

Last Updated: Jul 11, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Area of Science:

  • Epigenetics
  • Immunology
  • Molecular Biology

Background:

  • DNA hypomethylation is implicated in the development of systemic lupus erythematosus (SLE).
  • Various enzymes involved in DNA methylation are known, indicating cellular capacity for pattern modification.
  • Research on DNA methylation enzymes beyond DNMT1 in SLE is limited.

Purpose of the Study:

  • To analyze the simultaneous gene expression of key DNA methylation enzymes in SLE patients.
  • To deepen the understanding of the role of epigenetic mechanisms in SLE etiology.
  • To propose hypotheses for global DNA hypomethylation in CD4+ T cells of SLE patients.

Main Methods:

  • Review of existing literature on DNA methylation and SLE.
  • Investigation of simultaneous gene expression of DNMT1, DNMT3A, DNMT3B, MBD2, and MBD4 in SLE patients.
  • Analysis of DNA methylation patterns in CD4+ T cells.

Main Results:

  • Recent findings on the gene expression of DNA methylation enzymes in SLE patients are reported.
  • The study provides insights into the simultaneous expression patterns of DNMTs and MBDs.
  • Observed global DNA hypomethylation in CD4+ T cells of SLE patients.

Conclusions:

  • Further analysis of DNA methylation enzymes is essential for understanding SLE pathogenesis.
  • The findings contribute to understanding the epigenetic basis of SLE.
  • Alternative hypotheses are suggested to explain DNA hypomethylation in SLE CD4+ T cells.