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Updated: Jul 11, 2026

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
Published on: April 22, 2019
Ultrastructural study on the specific binding of genetically engineered epidermal growth factor to type I collagen
Koichi Kato1, Hideki Sato, Hiroo Iwata
1Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Kyoto, Japan.
Abstract:
In an attempt to develop collagen-growth factor composites for use in tissue engineering, chimeric proteins consisting of epidermal growth factor and collagen binding domains derived from von Willebrand factor or fibronectin were synthesized by means of recombinant technology. These chimeric proteins were bound to type I collagen fibrils, and the ultrastructures of composites were analyzed by transmission electron microscopy combined with the gold nanoparticle labeling technique. The results of the ultrastructural study revealed that chimeric proteins were densely assembled on collagen fibrils through the specific recognition of binding sites, producing the ordered array of chimeric proteins.
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