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Updated: Jul 11, 2026

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
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Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

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Functionalized carbon nanotubes for detecting viral proteins.

Yian-Biao Zhang1, Mandakini Kanungo, Alexander J Ho

  • 1Biology Department, Brookhaven National Laboratory, Building 463, Upton, New York 11973, USA.

Nano Letters
|September 27, 2007
PubMed
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Single-walled carbon nanotubes (SWNTs) functionalized with adenovirus Knob protein and its receptor CAR retain biological activity. These SWNT-CAR complexes show potential as sensitive biosensors for detecting adenoviruses.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Molecular Biology

Background:

  • Single-walled carbon nanotubes (SWNTs) offer unique electronic properties for biosensing applications.
  • Functionalizing SWNTs with biological molecules is crucial for developing specific and sensitive biosensors.
  • Understanding the impact of immobilization on protein activity is key for biosensor design.

Purpose of the Study:

  • To investigate the biocompatibility, specificity, and activity of a ligand-receptor-protein system covalently bound to SWNTs.
  • To establish a model proof-of-concept for using SWNTs as biosensors.
  • To evaluate the potential of SWNT-CAR complexes for detecting adenoviruses.

Main Methods:

  • Functionalization of SWNTs with adenovirus Knob protein or its receptor CAR via diimide-activated amidation.

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Last Updated: Jul 11, 2026

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
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Published on: January 10, 2017

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  • Confirmation of immobilized Knob protein activity using labeled conjugate antibodies.
  • Evaluation of bound CAR activity and specificity using fluorescently labeled Knob and a negative control protein (YieF).
  • Current-gate voltage (I-V(g)) measurements to assess protein binding effects on SWNT electronic properties.

    • Main Results:

    • Both Knob and CAR proteins fully retained their biological activities after immobilization on SWNT surfaces.
    • SWNT-CAR complexes demonstrated high sensitivity in detecting protein activity through electronic measurements.
    • The specificity of CAR binding to Knob was confirmed, indicating functional protein interactions.

    Conclusions:

    • Immobilized Knob and CAR proteins on SWNTs maintain their biological functions.
    • SWNT-CAR complexes exhibit potential as highly sensitive biosensors for environmental adenovirus detection.
    • This study provides a foundation for developing advanced SWNT-based biosensing platforms.