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Published on: May 21, 2015
[Detection of differentially expressed genes in oral lichen planus]
1Research Institute of Stomatology, Nanjing Medical University, Nanjing 210029, China.
Objective:
To detect the differentially expressed genes in oral lichen planus(OLP) by cDNA microarray.
Methods:
The PCR products of 4000 genes were spotted on chemical material coated glass plates in array. The total RNAs were isolated from OLP and normal oral mucosa tissue and were purified to mRNA. Both the mRNA were reversely transcribed to cDNA with the incorporations of fluorescent dUTP, for preparing the hybridization probes. The mixed probes were then hybridized to the cDNA microarray. Microarray was scanned for the fluorescent signals and showed the differences between the two samples.
Results:
The expressions of 122 genes were up regulated while the expressions of 91 genes were down regulated in OLP among the 4000 target genes. The up-regulated genes were mainly the ones of immunity related genes, metabolize related genes, oncogene, cytokine and cell signal transduction protein. The down-regulated genes were mainly the ones of DNA binding and transcription factors, cell signal transduction protein, immunity related genes, metabolize related genes, and cytokine.
Conclusion:
Two hundred and thirteen differentially expressed genes with different functions were revealed in OLP, which may play some roles in the progression of OLP.
Insights
This study identified 213 differentially expressed genes in oral lichen planus (OLP) using cDNA microarray analysis. These gene expression changes, including immune and metabolic pathways, offer insights into OLP development.
Area of Science:
- Genomics
- Oral Medicine
- Molecular Biology
Background:
- Oral lichen planus (OLP) is a chronic inflammatory condition affecting oral mucosa.
- Understanding the molecular mechanisms underlying OLP is crucial for developing effective treatments.
Purpose of the Study:
- To identify differentially expressed genes in oral lichen planus (OLP) tissues compared to normal oral mucosa.
- To elucidate the potential roles of these genes in OLP pathogenesis using cDNA microarray technology.
Main Methods:
- Utilized cDNA microarray analysis to screen 4000 genes.
- Isolated total RNA from OLP and normal oral mucosa tissues.
- Prepared fluorescently labeled cDNA probes for hybridization and signal detection.
Main Results:
- Identified 122 up-regulated and 91 down-regulated genes in OLP tissues.
- Up-regulated genes included immunity-related, metabolic, oncogenes, cytokines, and signal transduction proteins.
- Down-regulated genes involved DNA binding, transcription factors, and similar categories to up-regulated genes.
Conclusions:
- A total of 213 differentially expressed genes were identified in OLP.
- These genes, involved in various cellular functions, may contribute to the progression of OLP.
- The findings provide a molecular basis for further investigation into OLP.
