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Updated: Jul 11, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
[Mutations of short tandem repeat loci in Identifiler system]
Zhen-min Zhao1, Yan Liu, Yuan Lin
1Institute of Forensic Sciences, Ministry of Justice, PR China, Shanghai 200063, China. qzjdzx@163.com
Objective:
To explore and analyze the mutations of 15 Short Tandem Repeat (STR) loci using Identifiler system in paternity identification.
Methods:
2712 cases of paternity testing were carried out using Identifiler PCR Amplification Kit.
Results:
Of the 2362 paternity testing cases, mutations of single locus were observed in 51 cases. The mutation loci included D8S1179, D21S11, D7S820, CSF1PO, D3S1358, D13S317, D16S539, D2S1338, D19S433, vWA, D18S51, D5S818 and FGA, with the D21S11 locus having a highest mutation rate (0.369%). Thirty-six of the STR mutations were from paternal source, 7 from maternal source, and the rest (9) were undeterminable. The mutation rates at D21S11 were highest (0.369%).
Conclusion:
Mutations of STR loci are relatively common in human genome. Therefore, retesting of additional relatively stable STR loci with lower mutation rates is necessary when one or two loci exclusions are encountered in paternity testing.
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