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Updated: Jul 11, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
Published on: March 9, 2015
[Application of MiniFiler kit in forensic DNA testing of low copy number template]
Jian-ping Tang1, Dan Wu, Chen Zhang
1Institute of Forensic Science, Shanghai Municipal Public Security Bureau, Shanghai 200083, China.
Objective:
To detect low copy number of DNA samples by using a newly launched commercial miniSTR detection kit (MiniFiler) in forensic practice.
Methods:
Low concentration and/or challenged forensic DNA samples were analyzed according to protocols provided by the manufacturer (Applied Biosystems, Foster City, USA).
Results:
DNA samples as low as 10 pg could be amplified by MiniFiler kit, and the optimal DNA quantity was 40 pg or above.
Conclusion:
MiniFiler kit can be used for analysis of low copy number STR.

