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Updated: Jul 11, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
A surface immobilization method of endoglucanase from Cellulomonas biazotea mutant improved catalytic properties of
Muhammad Ibrahim Rajoka1, Yasmin Zia,
1Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan. mirajoka@nibge.org
Abstract:
Purified endoglucanase from C. biazotea mutant 51SM(r) was successfully immobilized on Eudragit L-100, with 75 % yield of immobilization. This method improved the kinetic and thermodynamic properties of the enzyme. Immobilization significantly decreased entropy and enthalpy of inactivation of biocatalyst and made it functionally and thermodynamically more stable and reusable compared to free one.

