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Lysosomes

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...

Lysosomes

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...

Lysosomal Hydrolases

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Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

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Related Experiment Video

Updated: Jul 11, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Integral and associated lysosomal membrane proteins.

Bernd Schröder¹, Christian Wrocklage¹, Cuiping Pan²

¹Institute of Physiological Chemistry, Philipps-University Marburg, Karl-von-Frisch-Straße 1, 35032 Marburg, Germany.

Traffic (Copenhagen, Denmark)

|September 28, 2007

Summary

Researchers identified 86 novel proteins in lysosomal membranes, including 12 with unknown functions and 16 enzymes and transporters. This discovery expands our understanding of lysosomal molecular transport and organelle interactions.

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Area of Science:

Cell Biology
Proteomics
Molecular Biology

Background:

Lysosomes are crucial organelles involved in cellular waste disposal and molecular degradation.
The precise protein composition of lysosomal membranes, particularly regarding molecular transport and inter-organelle communication, remains incompletely understood.

Purpose of the Study:

To identify novel proteins localized to lysosomal membranes.
To characterize proteins involved in molecular transport and interactions with other cellular compartments.
To expand the known proteome of the lysosomal membrane.

Main Methods:

Proteomic analysis of purified placental lysosomal membranes.
Identification of known and novel lysosomal membrane proteins.
Expression and subcellular localization studies of candidate novel proteins using fluorescent tagging in HeLa cells.

Main Results:

Identified 58 known lysosomal membrane proteins, including those associated with vacuolar adenosine triphosphatase.
Discovered 86 significantly enriched proteins in the lysosomal membrane fraction.
Characterized 12 novel proteins of unknown function, with two localized to lysosomes.
Detected 16 enzymes and transporters not previously assigned to lysosomal membranes.
Identified proteins involved in signaling and targeting associated with lysosomes.

Conclusions:

The study significantly expands the catalog of proteins found in lysosomal membranes.
Novel proteins with potential roles in molecular transport and cellular signaling have been identified.
This research provides a foundation for further investigation into lysosomal function and dynamics.