Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular Screening of Black Flies (Diptera: Simuliidae) for Vector-Borne Zoonotic Pathogens, South Moravia, Czech Republic.

Zoonoses and public health·2025
Same author

Human exposure to Dirofilaria immitis following a canine heartworm disease elimination program in Linosa Island (Sicily, Italy).

Acta tropica·2025
Same author

Respiratory and intestinal zoonotic cryptosporidiosis in symptomatic domestic pigeons (<i>Columba livia domestica</i>) in Tabriz, Iran.

Iranian journal of veterinary research·2025
Same author

Helminth parasites of impalas, <i>Aepyceros melampus</i>, in eastern southern Africa, collected during 1973 to 2007.

Journal of the South African Veterinary Association·2024
Same author

<i>Mansonella</i> sp. and associated <i>Wolbachia</i> endosymbionts in ring-tailed coatis (<i>Nasua nasua</i>) in periurban areas from Midwestern Brazil.

International journal for parasitology. Parasites and wildlife·2023
Same author

Molecular detection of Babesia spp. and Rickettsia spp. in coatis (Nasua nasua) and associated ticks from midwestern Brazil.

Parasitology research·2023

Related Experiment Video

Updated: Jul 11, 2026

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
10:09

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle

Published on: February 3, 2020

Comparative scanning electron microscopy of Gasterophilus third instars.

D D Colwell1, D Otranto, I G Horak

  • 1Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada. colwelld@agr.gc.ca

Medical and Veterinary Entomology
|September 28, 2007
PubMed
Summary

Scanning electron microscopy distinguished Gasterophilus species by cuticular features, except G. haemorrhoidalis and G. intestinalis, which were morphologically identical. This study clarifies feature functions and questions previous sensory structure descriptions.

More Related Videos

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
06:22

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

Published on: March 25, 2022

Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains
07:06

Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains

Published on: June 23, 2023

Related Experiment Videos

Last Updated: Jul 11, 2026

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
10:09

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle

Published on: February 3, 2020

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
06:22

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

Published on: March 25, 2022

Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains
07:06

Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains

Published on: June 23, 2023

Area of Science:

  • Veterinary Entomology
  • Comparative Morphology
  • Scanning Electron Microscopy

Background:

  • Accurate identification of Gasterophilus species is crucial for understanding their impact on livestock.
  • Previous taxonomic keys, such as Zumpt's compendium, provide foundational morphological data.
  • Gasterophilus larvae (bots) are significant internal parasites of equids.

Purpose of the Study:

  • To compare and differentiate third instar larvae of six Gasterophilus species using scanning electron microscopy.
  • To clarify the function of specific cuticular structures.
  • To identify reliable morphological markers for species identification.

Main Methods:

  • Comparative analysis of cuticular features using scanning electron microscopy (SEM).
  • Detailed examination of spine distribution and shape, maxillae, mandibles, cephalic sensillae, and terminal abdominal segments.
  • Microscopic imaging and morphological characterization of six Gasterophilus species.

Main Results:

  • Distinct morphological differences were observed among most Gasterophilus species examined.
  • Gasterophilus haemorrhoidalis and Gasterophilus intestinalis larvae were morphologically indistinguishable based on the features studied.
  • The function of certain cuticular structures, like respiratory chamber 'warts,' was clarified, and previously reported sensory structures were re-evaluated.

Conclusions:

  • SEM analysis provides valuable data for differentiating Gasterophilus species.
  • G. haemorrhoidalis and G. intestinalis require further investigation for distinct identification markers.
  • This research refines existing taxonomic knowledge and highlights areas for future study in Gasterophilus morphology.