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High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
Published on: May 10, 2016
Genotoxic effects of tanshinones from Hyptis martiusii in V79 cell line
B C Cavalcanti1, D J Moura, R M Rosa
1Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Cel Nunes de Melo, 1127, Caixa Postal-3157, 60430-270 Fortaleza, Ceará, Brazil.
Abstract:
The genotoxic effect of two tanshinones isolated from roots of Hyptis martiussi Benth (Labiatae) was studied using V79 (Chinese hamster lung) cells by the alkaline comet assay and micronucleus test. Tanshinones were incubated with the cells at concentrations of 1, 3, 6 and 12 microg/mL for 3 h. Tanshinones were shown to be quite strongly genotoxic against V79 cells at all tested concentrations. The data obtained provide support to the view that tanshinones has DNA damaging activity in cultured V79 cells under the conditions of the assays.
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