Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visual cycle-derived bisretinoids as endogenous natural products: enzyme-free formation, photochemical reactivity, and retinal degeneration.

Natural product reports·2026
Same author

Non-Erythropoietic EPO (EPO-R76E) Protects RPE Cells from Ferroptosis by Modulating the Labile Iron Pool and NRF2-GPX4 Axis.

Antioxidants (Basel, Switzerland)·2026
Same author

Chromophore-loaded CRALBP restores rod visual cycle in chromophore-deficient mice.

Molecular therapy. Advances·2026
Same author

Disease mechanisms revealed in the P23H opsin knock-in mouse model of retinitis pigmentosa.

PNAS nexus·2026
Same author

More insights from Abca4<sup>-/-</sup> mouse models of recessive Stargardt disease.

The Journal of biological chemistry·2026
Same author

Tracking Systemic and Ocular Vitamin A.

Cells·2026

Related Experiment Video

Updated: Jul 11, 2026

A Quick Phenotypic Neurological Scoring System for Evaluating Disease Progression in the SOD1-G93A Mouse Model of ALS
06:49

A Quick Phenotypic Neurological Scoring System for Evaluating Disease Progression in the SOD1-G93A Mouse Model of ALS

Published on: October 6, 2015

SOD2 knockdown mouse model of early AMD.

Verline Justilien1, Ji-Jing Pang, Kutralanathan Renganathan

  • 1Department of Molecular Genetics, University of Florida, Gainesville, Florida, USA.

Investigative Ophthalmology & Visual Science
|September 28, 2007
PubMed
Summary

Oxidative injury to the retinal pigment epithelium (RPE) can cause damage mimicking early age-related macular degeneration (AMD). This study shows that reducing manganese superoxide dismutase (MnSOD) in RPE cells leads to AMD-like changes in mice.

More Related Videos

Assessment of Spontaneous Alternation, Novel Object Recognition and Limb Clasping in Transgenic Mouse Models of Amyloid-&#946; and Tau Neuropathology
10:02

Assessment of Spontaneous Alternation, Novel Object Recognition and Limb Clasping in Transgenic Mouse Models of Amyloid-β and Tau Neuropathology

Published on: May 28, 2017

Intracerebroventricular Injection of Amyloid-&#946; Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits
08:01

Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits

Published on: March 16, 2016

Related Experiment Videos

Last Updated: Jul 11, 2026

A Quick Phenotypic Neurological Scoring System for Evaluating Disease Progression in the SOD1-G93A Mouse Model of ALS
06:49

A Quick Phenotypic Neurological Scoring System for Evaluating Disease Progression in the SOD1-G93A Mouse Model of ALS

Published on: October 6, 2015

Assessment of Spontaneous Alternation, Novel Object Recognition and Limb Clasping in Transgenic Mouse Models of Amyloid-&#946; and Tau Neuropathology
10:02

Assessment of Spontaneous Alternation, Novel Object Recognition and Limb Clasping in Transgenic Mouse Models of Amyloid-β and Tau Neuropathology

Published on: May 28, 2017

Intracerebroventricular Injection of Amyloid-&#946; Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits
08:01

Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits

Published on: March 16, 2016

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Molecular Biology

Background:

  • Oxidative stress is implicated in retinal diseases.
  • The retinal pigment epithelium (RPE) is vulnerable to oxidative damage.
  • Age-related macular degeneration (AMD) involves RPE dysfunction.

Purpose of the Study:

  • To investigate if oxidative injury to the RPE can induce retinal damage resembling early AMD.
  • To model AMD pathogenesis by targeting a key antioxidant enzyme in the RPE.

Main Methods:

  • Adeno-associated virus (AAV) delivered a ribozyme targeting manganese superoxide dismutase (MnSOD) into mouse retinas.
  • Analyzed RPE/choroid for MnSOD levels, oxidative damage markers, and bis-retinoids (A2E, iso-A2E).
  • Assessed retinal function using electroretinography (ERG) and examined cellular changes via microscopy.

Main Results:

  • Ribozyme treatment reduced MnSOD, increased oxidative damage markers, and induced RPE cell death.
  • Mice showed AMD-like features: RPE degeneration, Bruch's membrane thickening, photoreceptor damage, and increased A2E/iso-A2E.
  • ERG responses significantly decreased, indicating functional vision loss.

Conclusions:

  • Oxidative damage to the RPE is a potential contributor to AMD pathogenesis.
  • Targeting MnSOD in RPE cells can replicate key pathological features of early AMD.
  • This model provides insights into AMD mechanisms and potential therapeutic targets.