Oxidative burst induced pseudosclerotium formation of Morchella steppicola Zerova on different malt agar media

Morchella steppicola Zerova mycelia was grown on modified MS (MSK) medium and on MSK medium containing different malt content. Starvation induced an enhanced pseudosclerotium formation in a given zone of the culture. The malondialdehyde content of the mycelium at the site of pseudosclerotium formation was related to the acceleration of lipid peroxidation and oxidative stress. Superoxide dismutase activity was monitored in control and stressed vegetative hyphae subjected to malt withdrawal. In the formation zone of resting bodies, an enhanced oxidative burst was observed. The glucose and trehalose contents of M. steppicola mycelium during pseudosclerotium formation were also investigated. The glucose concentration of the mycelia approached a minimum during pseudosclerotium formation. At the same time, trehalose concentration reached a maximum in the zone of pseudosclerotium formation. The dry mass of mycelia in these zones was higher than average. The dry matter content of the pseudosclerotium-forming zones significantly exceeded the average of the whole culture. The correlation among trehalose accumulation, oxidative burst, and pseudosclerotium formation was clearly demonstrated.