Hyperglycemia and lipopolysaccharide decrease depression effect of interleukin 8 production by hypothermia: an experimental study with endothelial cells
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Akihiro Noda1, Kosaku Kinoshita, Atsushi Sakurai
1Department of Emergency and Critical Care Medicine, Nihon University School of Medicine, 30-1 Oyaguchi Kamimachi, Itabashi-ku, Tokyo 173-8610, Japan. anoda@med.nihon-u.ac.jp
Objective:
This study assessed whether hyperglycemia and lipopolysaccharide (LPS) decrease the depression effect of interleukin (IL) 8 production by hypothermia in endothelial cells.
Design And Setting:
Laboratory study in a university laboratory.
Subjects:
Human umbilical vein endothelial cells (HUVECs).
Interventions:
HUVECs were cultivated in various concentrations of glucose (5.5 or 16.5mM = 100 or 300mg/dl) with or without LPS stimulation for 5, 12, or 24h at either 30 degrees or 37 degrees C.
Results:
After culturing, IL-8 mRNA expressions and IL-8 levels were measured. At 37 degrees C, hyperglycemia significantly increased basal IL-8 mRNA at 12h and basal IL-8 at 24h. At 37 degrees C hyperglycemia significantly increased LPS-stimulated IL-8 mRNA at 12h and LPS-stimulated IL-8 at 12 and 24h. At 30 degrees C basal IL-8mRNA, basal IL-8, and LPS-stimulated IL-8 were significantly decreased by hypothermia, but these hypothermic effects were not observed in LPS-stimulated IL-8 mRNA. Furthermore even at 30 degrees C hyperglycemia significantly increased LPS-stimulated IL-8 mRNA at all time points and LPS stimulated IL-8 at 24h.
Conclusions:
Hypothermia (30 degrees C) decreases the production of IL-8 in HUVECs but does not decrease the expression of IL-8 mRNA. When hypothermia is followed by hyperglycemia and LPS stimulation, such a combination may expose the patients to a high risk of secondary tissue damage during therapeutic hypothermia.
