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Related Experiment Video

Updated: Jul 11, 2026

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

High tissue factor-expressing human monocytes carry low surface CD36: application to intersubject variability.

M A Sovershaev1, E M Egorina, F X Gruber

  • 1Department of Biochemistry, Institute of Medical Biology, University of Tromsø, Tromsø, Norway. mikhails@fagmed.uit.no

Journal of Thrombosis and Haemostasis : JTH
|September 29, 2007
PubMed
Summary

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High-responder individuals exhibit increased prothrombotic reactivity due to elevated tissue factor (TF) and reduced CD36 on monocytes. This monocyte phenotype may serve as a marker for identifying individuals prone to prothrombotic conditions.

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • The 'high and low responder' phenomenon describes variability in mononuclear cell (MNC) prothrombotic reactivity after lipopolysaccharide (LPS) stimulation.
  • Altered surface CD36 expression on monocytes is linked to impaired monocyte function.

Purpose of the Study:

  • To investigate the relationship between surface CD36 levels and monocyte prothrombotic reactivity in high-responder (HR) and low-responder (LR) individuals.
  • To determine if CD36 expression levels correlate with tissue factor (TF) presentation and prothrombotic activity.

Main Methods:

  • Compared TF mRNA, TF antigen, procoagulant activity, and surface CD36 expression in MNCs from HR and LR individuals.
  • Utilized flow cytometry to measure mean fluorescence intensities (MFIs) of surface CD36 and TF on CD14+ monocytes.

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Related Experiment Videos

Last Updated: Jul 11, 2026

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

On-Chip Endothelial Inflammatory Phenotyping
12:43

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

  • Analyzed correlations between surface TF and surface CD36 expression in resting and LPS-stimulated CD14+ cells.

    • Main Results:

    • Resting MNCs from HR individuals had higher TF mRNA but similar TF antigen and procoagulant activity compared to LR individuals.
    • Resting CD14+ monocytes from HR individuals showed significantly lower surface CD36 expression (MFI 70.4 vs. 132.0 AU).
    • Surface TF negatively correlated with surface CD36 in both resting (r = -0.598) and LPS-stimulated (r = -0.672) CD14+ cells.
    • LPS-stimulated MNCs from HR individuals exhibited significantly higher surface TF pools and procoagulant activity compared to LR individuals.
    • CD14+ monocytes from HR individuals displayed reduced surface CD36 during LPS challenge.

    Conclusions:

    • A novel monocyte phenotype characterized by high TF and low CD36 presentation was identified in HR individuals.
    • This phenotype may serve as a valuable marker for identifying individuals at higher risk for developing prothrombotic conditions.