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Related Experiment Video

Updated: Jul 11, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

High-performance liquid chromatography method for quantifying sphingomyelin in rat brain.

Diana J Azzam¹, Julnar A Usta, Youssef Mouneimne

¹Department of Biochemistry, Faculty of Medicine, American University of Beirut, Lebanon.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|September 29, 2007

Summary

A new high-performance liquid chromatography (HPLC) method accurately quantifies sphingomyelin in rat brains. This rapid and reproducible technique offers reliable results for sphingomyelin analysis.

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Last Updated: Jul 11, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

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Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Area of Science:

Biochemistry
Analytical Chemistry
Neuroscience

Background:

Sphingomyelin is a crucial sphingolipid in the central nervous system.
Accurate quantification of sphingomyelin is vital for understanding neurological disorders.

Purpose of the Study:

To develop and validate a rapid, reproducible, and accurate high-performance liquid chromatographic (HPLC) method for quantifying sphingomyelin in rat brain tissue.

Main Methods:

Utilized normal-phase silica gel chromatography with a specific solvent system (acetonitrile-methanol-water, 65:18:17 v/v).
Employed isocratic elution, UV detection at 207 nm, and 1,2-dimyristoyl-sn-glycero-3-phosphocholine as an internal standard.
Achieved a total run time of 10.0 minutes.

Main Results:

Demonstrated excellent linearity (R2>0.99) over the sphingomyelin concentration range of 0.025-0.4 mg/ml.
Reported low intra-day (1.4%-2.2%) and inter-day (3.1%) coefficients of variation, indicating high precision.
Established a practical limit of detection at 0.005 mg/ml and a quantification limit at 0.01 mg/ml.

Conclusions:

The developed HPLC method is suitable for the accurate and precise quantitative determination of sphingomyelin in rat brain samples.
This validated method provides a reliable tool for sphingomyelin research in neuroscience and related fields.