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Updated: Jan 9, 2026

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
Published on: March 4, 2021
Decoding protein modifications using top-down mass spectrometry
Nertila Siuti1, Neil L Kelleher
1Department of Chemistry, University of Illinois at Urbana-Champaign, 53 Roger Adams Laboratory, 600 South Matthews Avenue, Urbana, Illinois 61801, USA.
Abstract:
Top-down mass spectrometry is an emerging technology which strives to preserve the post-translationally modified forms of proteins present in vivo by measuring them intact, rather than measuring peptides produced from them by proteolysis. The top-down technology is beginning to capture the interest of biologists and mass spectrometrists alike, with a main goal of deciphering interaction networks operative in cellular pathways. Here we outline recent approaches and applications of top-down mass spectrometry as well as an outlook for its future.
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