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Ultrastructural analysis and identification of membrane proteins in the free-living amoeba Difflugia corona

Marcelo Silva-Briano1, Sandra Luz Martínez-Hernández, Araceli Adabache-Ortíz

  • 1Dept. of Biology, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes. Av. Universidad 940 C.P. 20100. Aguascalientes Ags, México.

Biocell : Official Journal of the Sociedades Latinoamericanas De Microscopia Electronica ... Et. Al
|October 2, 2007
PubMed

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Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...