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Related Experiment Videos

Starch removal from potato tuber sections.

A Fronda1, R Jona

  • 1Istituto di Coltivazioni Arboree, Facoltà di Agraria, Università di Torino, Italy.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|January 1, 1991
PubMed
Summary

A new method removes starch granules from plant sections before pectin removal. This prevents starch dextrinization, allowing accurate cell wall analysis using PAS staining and photometry.

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Area of Science:

  • Plant histology
  • Biochemistry
  • Microscopy techniques

Background:

  • Pectin removal requires heating plant sections with ammonium oxalate.
  • Heating starch-rich tissues causes dextrinization, interfering with cell wall analysis.
  • Accurate evaluation of cell wall extinction values is crucial in plant science.

Purpose of the Study:

  • To develop a method to prevent starch dextrinization during pectin removal.
  • To enable accurate photometric evaluation of cell wall staining in starch-rich plant tissues.

Main Methods:

  • A novel technique involves brushing away starch granules from plant sections after xylene treatment.
  • Sections are then heated to remove pectins.
  • Cell walls are stained using the Periodic Acid-Schiff (PAS) reaction.
  • Stain intensity is quantified using photometry.

Main Results:

  • The brushing method effectively removes starch granules.
  • Dextrinization of starch is significantly reduced or eliminated.
  • Photometric evaluation of PAS-stained cell walls becomes feasible and reliable.

Conclusions:

  • This simple brushing technique overcomes the challenge of starch dextrinization in plant histology.
  • It allows for precise quantification of cell wall components in starch-rich tissues.
  • The method enhances the accuracy of microscopic and spectrophotometric analyses in plant cell wall studies.

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