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Related Experiment Videos

Quantification of tartrate resistant acid phosphatase activity using a computerized image analysis system.

J D Kelly1, J A McNulty, G B Schneider

  • 1Department of Cell Biology, Neurobiology and Anatomy, Stritch School of Medicine, Loyola University of Chicago, Maywood, Illinois 60153.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|January 1, 1991
PubMed
Summary

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Tartrate resistant acid phosphatase (TRAP) reliably identifies osteoclasts. An image analysis method quantifies TRAP, differentiating osteoclasts from other TRAP-expressing cells like multinucleated giant cells (MNGCs).

Area of Science:

  • Biochemistry
  • Cell Biology
  • Histology

Background:

  • Tartrate resistant acid phosphatase (TRAP) is a recognized marker for osteoclast identification.
  • Distinguishing osteoclasts from other TRAP-expressing cells, such as multinucleated giant cells (MNGCs), is crucial for accurate biological interpretation.

Purpose of the Study:

  • To develop and validate an image analysis method for quantifying TRAP expression.
  • To differentiate osteoclasts from MNGCs based on TRAP expression levels.

Main Methods:

  • Utilized an image analysis system to quantitate TRAP.
  • Compared TRAP expression in osteoclasts versus MNGCs associated with implanted mineralized bone matrix in rats.
  • Analyzed TRAP demonstration in both implant sites and rat proximal tibiae.

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Main Results:

  • Osteoclasts consistently exhibited high TRAP expression.
  • MNGCs associated with bone implants showed minimal to no TRAP reaction product.
  • The image analysis system allowed for objective and reproducible quantification of TRAP.

Conclusions:

  • The developed image analysis method effectively quantifies TRAP, enabling differentiation between osteoclasts and MNGCs.
  • This quantitative approach provides a reliable tool for studying TRAP-expressing cells in various biological contexts.