Related Experiment Video
Updated: Jul 11, 2026
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
[Influence of the delay in urinary NMP22 preanalytical processing]
D Fatela-Cantillo1, A Fernández-Suárez, V Menéndez-López
1Area de Biotecnología, Centro Hospitalario de Alta Resolución Sierra del Segura, EPHAG, La Puerta de Segura-Puente de Génave, Jaén. danielfatela@yahoo.es
Objectives:
To evaluate the influence of the delay in urinary NMP22 preanalytical processing.
Material And Methods:
Twenty-eight voided urine samples were taken: bladder cancer (14), urine tract infections (4), lithiasis (4), healthy volunteers (1), and with other no malignant bladder diseases (5). All samples, were maintained at environment temperature, and were processed according to the stabilization of parts of urine collected at 0, 30, 90 and 150 minutes. Samples were stored at 4 degrees C until its determination. NMP22 was determined with the IMMULITE One analyzer.
Results:
There were no significant differences for NMP22 levels between each different point of time studied.
Conclusions:
Delay up to 2 hours and a half when we add stabilization solution to urine samples no affects NMP22 results. That thing, might provide more confidence and flexibility on quantitative immunoassays that required urine stabilization.
