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A high-affinity folate binding protein in human semen.

J Holm1, S I Hansen, M Høier-Madsen

  • 1Department of Clinical Chemistry, Central Hospital Nykøbing Falster, Denmark.

Bioscience Reports
|October 1, 1991
PubMed
Summary

Human semen contains a high-affinity folate binding protein (FBP). This protein

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Area of Science:

  • Reproductive Biology
  • Biochemistry

Background:

  • Folate binding protein (FBP) plays a crucial role in folate transport and metabolism.
  • While FBP is well-characterized in milk, its presence and characteristics in human semen are less understood.

Purpose of the Study:

  • To investigate the presence, characteristics, and concentration of folate binding protein (FBP) in human semen.
  • To determine the relationship between FBP concentration and sperm count.

Main Methods:

  • Equilibrium dialysis using radioligand 3H-folate to assess binding affinity and capacity.
  • Enzyme-linked immunosorbent assay (ELISA) with antibodies against human milk FBP to quantify immunoreactive FBP.
  • Gel filtration chromatography to determine the molecular weight of FBP in semen.

Main Results:

  • A high-affinity FBP (affinity constant 3.10(10) M-1) was detected in human semen.
  • Folate binding protein concentration in semen was independent of sperm count.
  • Two forms of FBP were identified: a major 100 kDa complex and a minor 25 kDa form.

Conclusions:

  • Human semen contains a high-affinity folate binding protein.
  • The concentration of this FBP is not directly related to sperm count.
  • Semen FBP exists in both monomeric (25 kDa) and potentially aggregated or complexed (100 kDa) forms.

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