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Updated: Jul 11, 2026
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
Published on: November 16, 2016
Effects of recombinant interferon-gamma on cytokine secretion from monocyte-derived macrophages infected with
Isil Fidan1, Emine Yesilyurt, Feryal Cetin Gurelik
1Faculty of Medicine, Department of Medical Microbiology, Gazi University, Dekanlik Binasi 2.Kat, Beşevler, Ankara 06500, Turkey. isilfidan@yahoo.com
Abstract:
Salmonella typhi (S. typhi) is an important pathogen which causes typhoid fever. The cytokines released from the macrophages, playing a role in the host defense against Salmonella infection, are crucial in the defense against the infection. IFN-gamma provides a protection against Salmonella infection by developing macrophage activation in different mechanisms. This study was designed to investigate the effect of the recombinant IFN-gamma (rIFN-gamma) on the cytokines secreted from S. typhi stimulated macrophages. Macrophage isolation was done in the heparinized blood samples obtained from healthy people, and following the priming with rIFN-gamma for 72h the cells were stimulated by S. typhi and then the cytokine levels in culture supernatants were determined by enzyme-linked immunosorbent assay. It was observed that rIFN-gamma reversely increased the levels of IL-1, IL-2 the levels of which were decreased by S. typhi and that it increased TNF-alpha levels while suppressing the levels of antiinflammatory cytokines such as IL-10 and TGF-beta the levels of which were increased by S. typhi. Consequently, rIFN was observed to increase protective Th1 response by affecting the secretion of cytokine during S. typhi infection and it was considered to be a good target especially to prevent and treat invasive Salmonella infections.
Insights
Recombinant interferon-gamma (rIFN-gamma) enhances protective immune responses against Salmonella typhi by modulating macrophage cytokine secretion. This suggests rIFN-gamma as a potential therapeutic target for invasive Salmonella infections.
Area of Science:
- Immunology
- Microbiology
- Infectious Diseases
Background:
- Salmonella typhi causes typhoid fever, a significant global health concern.
- Cytokines from macrophages are vital for host defense against Salmonella.
- Interferon-gamma (IFN-gamma) confers protection by activating macrophages.
Purpose of the Study:
- To investigate the impact of recombinant IFN-gamma (rIFN-gamma) on cytokine profiles of macrophages stimulated with S. typhi.
- To assess the potential of rIFN-gamma in modulating the immune response to S. typhi infection.
Main Methods:
- Macrophage isolation from healthy donors' blood.
- Priming macrophages with rIFN-gamma for 72 hours.
- Stimulation of macrophages with S. typhi and subsequent cytokine level determination via ELISA.
Main Results:
- rIFN-gamma reversed S. typhi-induced decreases in IL-1 and IL-2 levels.
- rIFN-gamma elevated TNF-alpha levels.
- rIFN-gamma suppressed anti-inflammatory cytokines (IL-10, TGF-beta) elevated by S. typhi.
Conclusions:
- rIFN-gamma promotes a protective Th1 immune response by altering cytokine secretion during S. typhi infection.
- rIFN-gamma shows promise as a therapeutic strategy for invasive Salmonella infections.
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