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Related Experiment Video

Updated: Jul 11, 2026

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

Published on: March 31, 2012

FGF mediated Sulf1 regulation.

Wanfeng Zhao¹, Steve Allen, Gurtej K Dhoot

¹Department of Basic Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK.

|October 2, 2007

Summary

This summary is machine-generated.

Fibroblast growth factor 4 (FGF4) regulates Sulf1 during embryonic limb development. FGF4 influences cell death and digit formation, with Sulf1 acting as a key mediator in these processes.

Related Experiment Videos

Last Updated: Jul 11, 2026

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

Published on: March 31, 2012

Area of Science:

Developmental Biology
Molecular Biology
Signaling Pathways

Background:

Fibroblast Growth Factor (FGF) signaling is essential for embryonic development.
Sulf1 is known to antagonize FGF activity.
The specific role of FGF4 in Sulf1 regulation during limb development requires further elucidation.

Purpose of the Study:

To investigate the regulatory role of FGF4 on Sulf1 expression during quail autopod (limb bud) development.
To understand how FGF4 affects apoptosis and digit formation in relation to Sulf1.

Main Methods:

Implantation of FGF4-soaked beads into quail embryonic limb buds (interdigit and digit tip).
Micromass cultures to assess Sulf1 regulation in vitro.
Administration of FGF inhibitor SU5402 to examine its effects on Sulf1 expression and digit development.

Main Results:

FGF4 beads differentially upregulated Sulf1 expression in both interdigital and digital tip regions.
FGF4 demonstrated a concentration-dependent inhibition of apoptosis in the interdigital mesoderm.
The FGF inhibitor SU5402 suppressed Sulf1 expression in the interdigit but induced ectopic Sulf1 expression and inhibited phalange formation at the digital tip.

Conclusions:

FGF4 plays a significant role in regulating Sulf1 expression during embryonic limb development.
Sulf1 is implicated in mediating FGF4's effects on interdigital apoptosis and digit formation.
The precise localization of FGF signaling and Sulf1 activity is critical for normal phalange development.