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Updated: Jul 11, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
NADH dehydrogenase subunits are overexpressed in cells exposed repeatedly to H2O2
Rita Ghosh1, Koyeli Girigoswami
1Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, Nadia, India. ritadg2001@yahoo.co.in
Abstract:
Cells conditioned by repeated treatments with low doses of H(2)O(2,) were compared with its parental V79 cells for expression of ND1 and ND4 subunits of NADH dehydrogenase, a mitochondrial gene. It was found that ND1 and ND4 subunits were overexpressed in these conditioned cells. These cells were also found to be resistant to killing upon gamma-irradiation through suppression of apoptotic cell death. On irradiation, the expression of both subunits decreased in both cell types, but overall there was more expression of both subunits in the conditioned cells. These findings indicate alteration in the expression of NADH dehydrogenase, a mitochondrial gene, could be involved in the recovery of gamma-irradiated cells through inhibition of apoptosis.
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