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Updated: Jul 11, 2026

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
07:29

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

cDNA representational difference analysis used in the identification of genes expressed by Trichophyton rubrum during

Lilian Cristiane Baeza1, Alexandre Melo Bailão, Clayton Luiz Borges

  • 1Laboratório de Micologia Clínica, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, CEP 14801-902, 1621 Araraquara, SP, Brazil.

Microbes and Infection
|October 2, 2007
PubMed

Insights

This study identified genes in Trichophyton rubrum that are activated when the fungus encounters keratin, aiding its infection of human skin. These findings enhance understanding of dermatophyte infections and potential therapeutic targets.

Area of Science:

  • Mycology
  • Molecular Biology
  • Dermatology

Background:

  • Dermatophytes, like Trichophyton rubrum, infect skin, hair, and nails by metabolizing keratin.
  • Trichophyton rubrum causes the majority of chronic dermatophytosis cases.
  • Understanding fungal gene expression during infection is crucial for host-pathogen interaction studies.

Purpose of the Study:

  • To identify genes expressed by Trichophyton rubrum during keratin utilization and host interaction.
  • To elucidate the molecular mechanisms underlying dermatophyte infections.

Main Methods:

  • Representational difference analysis (RDA) was used to compare gene expression between T. rubrum cultured with keratin and in minimal medium.
  • Differentially expressed genes were identified.
  • Real-time PCR validated the expression patterns in vitro using human keratinocytes.

Main Results:

  • Genes involved in signal transduction, membrane proteins, oxidative stress response, and virulence factors were upregulated upon contact with keratin.
  • Expression patterns confirmed the role of these genes in the infective process.
  • Identified genes provide insights into T. rubrum's adaptation and pathogenicity.

Conclusions:

  • The study identified key genes crucial for Trichophyton rubrum's ability to infect keratinized tissues.
  • Findings contribute to a deeper understanding of dermatophytosis pathogenesis.
  • This knowledge may inform the development of novel antifungal strategies.

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