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Related Concept Videos

Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Organization01:13

Protein Organization

Overview
Protein Organization01:13

Protein Organization

Overview
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Folding01:22

Protein Folding

Overview
Protein Folding01:22

Protein Folding

Overview

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Related Experiment Video

Updated: Jul 11, 2026

A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

M-TASSER: an algorithm for protein quaternary structure prediction.

Huiling Chen1, Jeffrey Skolnick

  • 1Center for the Study of Systems Biology, School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA.

Biophysical Journal
|October 2, 2007
PubMed
Summary
This summary is machine-generated.

Predicting protein quaternary structure is challenging due to limited solved structures. M-TASSER, a new method, accurately predicts protein complex structures from sequence, even with weak template similarity.

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Quaternary Structure Modeling Through Chemical Cross-Linking Mass Spectrometry: Extending TX-MS Jupyter Reports

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Area of Science:

  • Structural Biology
  • Computational Biology
  • Biophysics

Background:

  • Proteins interact extensively, forming complexes crucial for cellular functions.
  • A vast number of protein interactions are known or suspected, but few have solved structures.
  • Predicting protein quaternary structure is vital for understanding biological mechanisms.

Purpose of the Study:

  • To develop a computational method for predicting protein quaternary structure from amino acid sequence.
  • To address the challenge of predicting structures for proteins with weak sequence similarity to known complexes.
  • To improve the accuracy of protein complex structure prediction.

Main Methods:

  • Developed M-TASSER, a hierarchical prediction method.
  • Employed multimeric threading for template identification.
  • Utilized multimer model assembly and refinement, with structure clustering for final model selection.

Main Results:

  • M-TASSER successfully predicted quaternary structures for 80% of tested dimer targets.
  • Achieved a true positive rate of 68% and a false positive rate of 17%.
  • Refined models showed significant improvement in accuracy (1.3-1.5 Å RMSD reduction) over initial templates.

Conclusions:

  • M-TASSER is a promising approach for predicting protein quaternary structure.
  • The method is effective even for proteins with weak sequence similarity to known structures.
  • Accurate prediction of protein complex structures aids in understanding protein function and biological pathways.