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Escherichia coli physiology in Luria-Bertani broth
Guennadi Sezonov1, Danièle Joseleau-Petit, Richard D'Ari
1Unité Biologie Moléculaire du Gène chez les Extrémophiles, Institut Pasteur, 25, rue du Docteur Roux, F-75724 Paris Cedex 15, France. sezonov@pasteur.fr
Journal of Bacteriology
|October 2, 2007
Abstract:
Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD(600)) of 7. Surprisingly, however, steady-state growth ceases at an OD(600) of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars.
