Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein-sphingolipid interactions within cellular membranes.

Per Haberkant1, Oliver Schmitt, F-Xabier Contreras

  • 1Heidelberg University Biochemistry Center, 69120 Heidelberg, Germany.

Journal of Lipid Research
|October 2, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy.

Signal transduction and targeted therapy·2026
Same author

DGAT-driven futile lipid cycling has a pronounced, yet concealed, thermogenic function.

Cell metabolism·2026
Same author

Suggested experimental design and computational modeling to infer single-cell lipid dynamics from a single destructive measurement.

iScience·2026
Same author

Alkyne lipids as tracers of lipid metabolism.

Methods in enzymology·2026
Same author

Cholesterol-lowering effects of oats induced by microbially produced phenolic metabolites in metabolic syndrome: a randomized controlled trial.

Nature communications·2026
Same author

DGAT-driven futile lipid cycling has a pronounced, yet concealed, thermogenic function.

Cell metabolism·2026
Same journal

Reevaluation of mycobacterial Phosphatidyl-myo-Inositol Mannosides (PIMs) as Toll-Like Receptor 2 (TLR2) Agonists.

Journal of lipid research·2026
Same journal

Erratum to 'Stearoyl-CoA desaturase-1 is vital for milk lipid synthesis: Deletion impairs mammary gland and neonatal development' [Journal of Lipid Research 66/12 (2025) 100941].

Journal of lipid research·2026
Same journal

The Metabolic-Immune Axis in MASH: Is the Macrophage Signature the Key to Liver Disease Staging?

Journal of lipid research·2026
Same journal

BK channel deficiency promotes lipolysis via an AKT-independent activation of the cAMP/PKA/HSL pathway.

Journal of lipid research·2026
Same journal

Interleukin-6 stimulates platelet 12-lipoxygenase to drive coagulation in inflammatory arthritis.

Journal of lipid research·2026
Same journal

LIPA regulates myeloid differentiation and is essential for intra-plaque macrophage accumulation during atherogenesis.

Journal of lipid research·2026
See all related articles

Researchers developed a novel photoactive sphingolipid probe to study how lipids interact with transmembrane proteins in living cells. This tool helps reveal specific protein-lipid interactions critical for membrane organization and protein function.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Membrane Biophysics

Background:

  • Intracellular organelles require precise lipid compositions for membrane function and biophysical properties.
  • Understanding molecular mechanisms of protein targeting and segregation within membrane microdomains is crucial.
  • The specific lipid environment of transmembrane segments and its impact on protein function remain largely unknown.

Purpose of the Study:

  • To develop a novel tool for investigating specific protein transmembrane span-lipid interactions in vivo.
  • To elucidate the role of lipid environments in protein conformation, activity, and regulation within membrane microdomains.

Main Methods:

  • Synthesis of a novel photoactive sphingolipid precursor.
  • In vivo investigation of protein-lipid interactions using the sphingolipid probe, phosphoglycerolipid precursor, and photo-cholesterol.

Related Experiment Videos

  • Demonstration of specific labeling of the ceramide transporter and interactions with caveolin-1, phosphatidylinositol transfer protein beta, and mature nicastrin.

    • Main Results:

    • Successful synthesis and application of a novel photolabile sphingolipid probe.
    • Specific in vivo labeling of the ceramide transporter was achieved.
    • Specific lipid interactions with caveolin-1, phosphatidylinositol transfer protein beta, and mature nicastrin were identified.

    Conclusions:

    • The novel photolabile sphingolipid probe enables the detection of protein-sphingolipid interactions within the membrane bilayer of living cells.
    • This technology provides a new avenue for studying the functional relevance of specific lipid environments for transmembrane proteins.