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Updated: Jul 11, 2026
07:22
Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes
Published on: June 2, 2020
[Physiological function of osteocytes]
1National Center for Geriatrics and Gerontology, Department of Bone and Joint Disease.
Clinical Calcium
|October 2, 2007
Summary
Osteocytes regulate phosphate and mineralization. Dentin matrix protein 1 (DMP1) mutations increase fibroblast growth factor 23 (FGF23), suggesting DMP1 negatively controls FGF23 in bone.
Area of Science:
- Bone Biology
- Endocrinology
- Mineral Metabolism
Background:
- Osteocytes are key bone cells producing DMP1, FGF23, and sclerostin.
- FGF23 regulates phosphate homeostasis and connects bone and kidney.
- DMP1 is crucial for bone mineralization.
Purpose of the Study:
- To investigate the role of DMP1 in regulating FGF23 production by osteocytes.
- To explore the function of osteocytes in bone remodeling and response to mechanical stimuli.
Main Methods:
- Analysis of patients with DMP1 mutations.
- Utilizing a targeted osteocyte ablation mouse model.
Main Results:
- DMP1 mutations are associated with elevated FGF23 and hypophosphatemia.
- Osteocyte ablation models reveal roles in bone remodeling and mechanical response.
Conclusions:
- DMP1 negatively regulates FGF23 in osteocytes.
- Osteocytes play critical roles in bone physiology and pathology.
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