Hydrogen peroxide in the eye lens: radioisotopic determination

Lenses from normal rabbits, mice, rats, cattle, guinea pigs, lambs, chicken, cats, baboons, blue acara (fish) and dogs were examined for the presence of H2O2. No previous reports exist on the presence and levels of H2O2 in normal eye lenses. Freshly isolated lenses of these animals were extracted with trichloroacetic acid and the extract neutralized with Tris. H2O2 was assayed in these extracts by reacting them with 1-14C-alpha-ketoglutarate and measuring the 14CO2 produced by peroxide-dependent decarboxylation. Peroxide of the order of 10(-4)M was detected in most of the lenses except in baboons wherein it exists between 10(-4) and 10(-5)M. Culture experiments with rat lenses demonstrated that GSH may make a major contribution to the formation of H2O2 in the intact lens in vivo.