Antibiotic susceptibility and outer membrane proteins of clinical Xanthomonas maltophilia isolates
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1Department of Medical Microbiology and Immunology, Ruhr-Universität, Bochum, FRG.
Abstract:
Twenty clinical Xanthomonas maltophilia isolates were studied for their susceptibility to various antibiotics and for their outer membrane protein profiles and compared to Pseudomonas aeruginosa isolates. Among the antibiotics studied, fleroxacin and ciprofloxacin exhibited the greatest activity (MIC90 4 micrograms/ml). All the X. maltophilia isolates exhibited similar outer membrane protein profiles with 40, 23 and 21 major proteins, whereas the outer membrane of P. aeruginosa revealed at least 7 major outer membrane proteins, as is known from the literature. Thus, it is assumed that the outer membrane profile of X. maltophilia contributes to the resistance of this nosocomial pathogen to most antibiotics.
Insights
Xanthomonas maltophilia exhibits resistance to many antibiotics, potentially due to its unique outer membrane protein profile. Fleroxacin and ciprofloxacin showed the most activity against these clinical isolates.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Xanthomonas maltophilia is a significant nosocomial pathogen.
- Understanding its antibiotic resistance mechanisms is crucial for effective treatment.
- Outer membrane proteins play a role in bacterial resistance.
Purpose of the Study:
- To investigate the antibiotic susceptibility of clinical Xanthomonas maltophilia isolates.
- To analyze the outer membrane protein (OMP) profiles of X. maltophilia.
- To compare these characteristics with Pseudomonas aeruginosa.
Main Methods:
- Susceptibility testing against various antibiotics.
- Outer membrane protein profiling using SDS-PAGE.
- Comparison of OMP profiles between X. maltophilia and P. aeruginosa.
Main Results:
- Fleroxacin and ciprofloxacin demonstrated the highest activity against X. maltophilia (MIC90 = 4 µg/mL).
- All studied X. maltophilia isolates shared similar OMP profiles with 40, 23, and 21 major proteins.
- Pseudomonas aeruginosa exhibited at least 7 major outer membrane proteins.
Conclusions:
- The distinct outer membrane protein profile of X. maltophilia is hypothesized to contribute to its broad antibiotic resistance.
- This finding highlights potential targets for novel antimicrobial strategies against this pathogen.