Effect of bleaching on the cemento-enamel junction
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Updated: Jul 11, 2026

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Published on: March 27, 2026
Roberta Esberard1, Renata R Esberard, Roberto M Esberard
1Department of Pathology, Bauru Dental School, University of São Paulo, Brazil. roesberard@hotmail.com
Purpose:
To evaluate the effect of various bleaching agents on the cemento-enamel junction (CEJ) of human teeth by scanning electron microscopy (SEM) analysis.
Methods:
30 intact teeth were selected and longitudinally sectioned, yielding 60 specimens. Thirty specimens served as controls; the other 30 were divided into six groups with five specimens each (n = 5) and bleached according to six protocols (Group 1: External bleaching with 10% carbamide peroxide; Group 2: External bleaching with 35% hydrogen peroxide; Group 3: External bleaching with 35% hydrogen peroxide; Group 4: Internal/external bleaching with 35% hydrogen peroxide; Group 5: Internal/external bleaching with 35% hydrogen peroxide; and Group 6: Intracoronal bleaching with a paste of sodium perborate mixed with 9% hydrogen peroxide). After treatment the specimens were prepared and examined in a scanning electron microscope.
Results:
The bleaching agents used in this study caused morphological changes in the CEJ and increased dentin exposure.
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