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Updated: Jul 11, 2026
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
Published on: May 16, 2012
Detection of pim-1 mRNA in prostate cancer diagnosis
Hui-chan He1, Xue-cheng Bi, Qi-shan Dai
1Department of Urology, First People's Hospital of Guangzhou, Guangzhou Medical College, Guangzhou 510180, China.
Background:
Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa.
Methods:
The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve.
Results:
The ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively).
Conclusion:
Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.
