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Updated: Jul 11, 2026
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
Published on: June 22, 2016
[Immature dendritic cells directional differentiated from murine hemopoietic stem cells and morphous and function of
Ya-Zhuo Jiang1, Pu-Xun Tian, Xiao-Ming Ding
1Department of Renal Transplantation, the First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China. jerry_jyz@yahoo.com.cn
Aim:
To Investigate a method that can obtain massive highly purified immature dendritic cells(imDCs) steadily in vitro, and identify them by morphous, function and surface markers by MACS.
Methods:
Isolate and purify CD117(+) hemopoietic stem cells(HSCs) from bone marrow of healthy C57 murine by MACS. After being expanded by SCF+IL-3, HSCs would be directional differentiated into imDCs by use of cytokine scheme of GM-CSF+IL-4+IL-10. Then identify imDCs through following ways: observing morphous and function of them under inverted microscope, scanning electron microscope and transmission electron microscope, detecting the expression of surface markers by flow cytometry.
Results:
The fold of expansion 3, 5 and 7 days after cultured with SCF+IL-3 was separately 10.34 +/- 1.43, 22.65 +/- 2.71 and 54.39 +/- 3.08. HSCs can be successfully differentiated into imDCs, which have the function of phagocytosis. The imDCs were short and small, in shape of sentus. The expression of surfacee markers was CD11c(+), I-A/I-E(low), CD40(-), CD80(-), CD86(-) by flow cytometry.
Conclusion:
This method can obtain and identify massive highly purified imDCs steadily in vitro.
