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[A dual effect on the growth of astrocytes of lipopolysaccharide]
Xue-Zhong Li1, Yuan Zhou, Chun-Feng Liu
1Department of Neurology, the Second Affiliated Hospital, Soochow University, Suzhou, China. sudalxz@163.com
Aim:
To investigate the changes of growth of astrocytes administrated with lipopolysaccharide (LPS) and the probable mechanisms.
Methods:
LPS was administrated to rat astrocytes for 1 h, 6 h, 24 h and 48 h, and the changes of growth of astrocytes were detected by MTT method. Meanwhile, the long-term changes of growth of astrocytes after administration with LPS for 24 h were also studied. Moreover, the effects of inhibitor of NF-kappaB(SN50) on proliferation of astrocytes were observed.
Results:
Changes of astrocytes growth were observed only when LPS had been administrated for 24 h. LPS of low concentration could promote proliferation of astrocytes and increase the cell viability(P<0.05 vs control), while LPS of high concentration inhibited the proliferation of astrocytes. Being administrated for 24 h, LPS could promote the proliferation of astrocytes in short-term. As for the long-term effects, LPS inhibited the proliferation of astrocytes in a concentration-dependent manner. Pretreatment with SN50 could block the effects of LPS on astrocytes.
Conclusion:
LPS of low concentration could promote the proliferation of astrocytes in short-term and LPS of high concentration could inhibit it. The molecular mechanism of the changes might be related to the activation of NF-kappaB.
