Related Experiment Video
Updated: Jul 11, 2026
10:59
Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands
Published on: July 26, 2014
[3D Histology: a protocol of micrographic surgery well fitted for French dermatologists and pathologists]
M Moehrle1, H Breuninger, A Taïeb
1Service de Dermatologie, Universitäts-Hautklinik, Eberhard-Karls-Universität, Liebermeisterstr. 25, D-72076 Tübingen, Allemagne. matthias.moehrle@med.uni-tuebingen.de
Annales De Pathologie
|October 3, 2007
Abstract:
We present 2 techniques of micrographic surgery (3D-histology) useful to control lateral and deep limits of cutaneous tumors. We have adapted the protocol created in Germany, at the University of Tuebingen, for French pathologists and dermatologists.
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The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...