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Updated: Jul 11, 2026
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High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Published on: November 28, 2016
Prevotella nanceiensis sp. nov., isolated from human clinical samples
C Alauzet1,2, F Mory1, J-P Carlier3
1Centre Hospitalier et Universitaire de Nancy, Hôpital Central, Laboratoire de Bactériologie, Nancy, France.
Summary
Three novel anaerobic bacteria, Prevotella nanceiensis, were identified from human samples. These Gram-negative bacilli represent a distinct species within the Prevotella genus based on genetic and phenotypic analyses.
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- Human clinical samples can harbor diverse anaerobic bacteria.
- Accurate bacterial identification is crucial for understanding infection and disease.
Purpose of the Study:
- To characterize three previously unidentified anaerobic, non-pigmented, Gram-negative bacilli.
- To determine if these isolates represent a novel species within the Prevotella genus.
Main Methods:
- Phenotypic characterization including metabolic end products.
- Genotypic analysis using 16S rRNA and rpoB gene sequencing.
- Phylogenetic analysis and DNA G+C content determination.
Main Results:
- The three strains formed a distinct homogeneous group, closely related to Prevotella marshii and Prevotella shahii.
- 16S rRNA identity was 89.8% and 89.0%, rpoB gene identity was 83.1% and 82.8%.
- Saccharolytic activity and production of acetic, lactic, and succinic acids were observed.
Conclusions:
- The isolates represent a novel species, Prevotella nanceiensis sp. nov.
- Polyphasic analysis supports the distinctiveness of this new Prevotella species.
- Strain LBN 293(T) is proposed as the type strain for Prevotella nanceiensis.
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