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Updated: Jul 11, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

MAGED2: a novel p53-dissociator

Chris Papageorgio1, Rainer Brachmann, Jue Zeng

  • 1Hematology and Medical Oncology, Ellis Fischel Cancer Center, University of Missouri-Columbia, Columbia, MO 65203, USA. papageorgioc@health.missouri.edu

International Journal of Oncology
|October 4, 2007
PubMed

Insights

Researchers identified MAGED2 as a novel protein that negatively regulates wild-type p53 activity. MAGED2 interacts with p53, affecting its function in cancer cells and tissues.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Protein Interactions

Background:

  • The tumor suppressor protein p53 is a critical transcription factor frequently mutated in human cancers.
  • Wild-type p53 (wt p53) is activated by post-transcriptional modifications upon DNA damage, inducing apoptosis or cell cycle arrest.

Purpose of the Study:

  • To identify novel regulators of wild-type p53 activity.
  • To investigate the role of MAGED2 in modulating p53 function.

Main Methods:

  • Yeast p53-dissociator assay to identify potential regulators.
  • Co-immunoprecipitation and reporter gene assays in human cultured cells.
  • Analysis of p53 and MAGED2 co-expression in human cancer tissue microarrays.

Main Results:

  • MAGED2 was identified as a potential negative regulator of wt p53.
  • MAGED2 physically interacts with p53 and modifies its activity in human cells.
  • Co-expression of p53 and MAGED2 was observed in the nucleus and cytoplasm of 2,682 human cancer specimens.

Conclusions:

  • MAGED2 acts as a novel p53-dissociator, negatively regulating wt p53 activity.
  • The interaction between MAGED2 and p53 has implications for cancer biology.
  • MAGED2 represents a potential target for cancer therapy.

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